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Immunoblots of cell lysates from spirochetes grown at pH 7.0 and 8.0 (A), E. coli DH5α recombinant clone 18c with control (B), and E. coil DH5α transformed with pCR2.1-A36 with control (C) probed with hyperimmune serum cross-adsorbed with cell lysates from E. coli and B. burgdorferi B31 grown at pH 8.0. The asterisk indicates the 25-kDa immunoreactive protein identified as the gene product of bba36. Molecular mass standards in kilodaltons are indicated on the left.
