Figure 2.

Activation of the androgen receptor leads to a downregulation of the NF-κB activity in VCAP and LNCAP cells. (A–D) Luciferase assays for control (androgen-depleted) and DHT-treated VCAP and LNCAP cells using reporter plasmids for androgen receptor (AR) as well as NF-κB transcriptional activity. (E,F) DNA binding activity of NF-κB assessed by ABCD assays as alternative to EMSAs. β-tubulin was used as a loading control. (G,H) Quantification of (E,F). Statistical analysis was performed by two-tailed Fisher’s exact test (n = 5; error bars represent SEM, * p < 0.05; ** p < 0.01, *** p < 0.001). (I,J) Protein levels of p65 and p50 NF-κB forms of samples shown in (E,F). The uncropped Western blots have been shown in Figure S5.