Figure 3.

Antiandrogen treatment of LNCaP cells upregulates NF-κB signaling and reduces cell death of prostate cancer cells. A public microarray dataset of LNCaP cells treated with the antiandrogen bicalutamide (GEO database, GSE56188) was reanalyzed with the Ingenuity Pathway Analysis software. (A) Depiction of canonical pathways, showing NF-κB signaling significantly enhanced (orange color reflects a positive z-score, indicated by a black box). (B) Disease/function analysis implying that apoptosis, as well as necrosis, of prostate cancer cell lines is reduced, as reflected by the blue color (indicating a negative activation z-score), while cell death of most other cells is elevated. Lower panel: effect on proliferation of cells. Significantly altered biological functions are plotted (using the ggplot2 package of R Bioconductor) with negative log-p-values on the x-axis.