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. 2022 Dec 14;14(24):6164. doi: 10.3390/cancers14246164

Figure 5.

Figure 5

Immune-fluorescence microscopy of AR and p65 in castrated versus sham-operated mice. (A) Representative fluorescence microscopy images of prostate tissue sections from C57BL/6 mice (castrated or sham-operated) stained for AR, p65/RelA (NF-κB), and DAPI (for DNA and cell recognition). The right panel shows the cell masks detected with the IKOSA-image analysis platform (see Section 2) The scale bar indicates 250 µm. Samples from castrated mice (castr.) comprised 6 sections, from which 33 view fields were captured for the three fluorescence microscopy channels. Cell recognition resulted in the detection of 30,988 cells. Samples from sham-operated mice comprised 7 sections, 29 view fields, and resulted in the detection of 27,665 cells. (B) Quantification of cellular fluorescence intensities for AR, p65, and DAPI (as control) for sham-operated and castrated mice; median values with error bars representing the 95% confidence interval. Statistical analysis was performed with a nonparametric Mann–Whitney test (**** p < 0.0001). (C) Quantification of nuclear to cytosolic fluorescence for AR and p65 (images from A, median values with error bars representing the 95% confidence interval, **** means p < 0.0001).