Table 3.
Variants detected by NGS analysis in paired tumor and plasma samples.
| PATIENT No. | GENE | MUTATION | VAF % TUMOR | VAF% PLASMA |
|---|---|---|---|---|
| 1 | MET | T1010I | 58.89 | 49.53 |
| 2 | MET KRAS |
c.3082 + 2T > C p.? G13D |
- - |
0.81 0.51 |
| 3 | MET TP53 |
exon 14 Skipping c.2942-11_2986del p.? R213 * V143M |
- 23.31 1.40 - |
- - 0.80 0.65 |
| 4 | SF3B1 TP53 |
K700E G244D |
- - |
0.25 0.52 |
| 5 | FBXW7 TP53 |
R505S R248W |
33.1 73.01 |
3.46 5.07 |
| 6 | - | - | - | - |
| 7 | PIK3CA | E545K | 18.3 | 5.28 |
| 8 | TP53 | [E271D; V272L] V272L |
5.08 5.22 |
1.07 - |
| 9 | TP53 | R282G | 46.79 | 4.14 |
| 10 | FGFR3 PTEN TP53 |
R248C D24Y C141W |
51.35 64.07 - |
22.61 20 16.24 |
| 11 | TP53 | C242Y | 36.3 | 1.74 |
| 12 | EGFR TP53 |
L858R R248W V197M |
90.99 41.62 - |
23.62 2.33 0.26 |
| 13 | - | - | - | - |
| 14 | KRAS TP53 |
G12C G245S R213P |
33.77 42.3 - |
1.89 0.88 1.26 |
| 15 | - | - | - | - |
| 16 | TP53 | S303Afs*42 | 32.71 | 2.02 |
| 17 | EGFR | L858R | 22.19 | 6.52 |
| 18 | EGFR | L858R | 84.25 | 3.61 |
| 19 | BRAF EGFR |
V600E L858R |
3.06 - |
0.26 0.26 |
| 20 | KRAS | G12C | 33.46 | 0.16 |
| 21 | KRAS TP53 |
G12V R213L |
18.36 11.61 |
3.40 2.23 |
| 22 | KRAS EGFR |
G12D Q61H L858R |
12.81 - - |
0.12 0.57 0.37 |
| 23 | NRAS TP53 |
Q61L G266 * R273H |
34.53 23.48 - |
4.72 2.89 0.82 |
| 24 | - | - | - | - |
| 25 | TP53 | S215I | 13.69 | 0.11 |
| 26 | MET | c.3082 + 1 G > C p.? | - | 11.68 |
*—stop codon.