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. 2022 Dec 8;11(24):3975. doi: 10.3390/cells11243975

Figure 1.

Figure 1

The design of MAIO-epiCBE system for base editing of the PERV genome. (A) Schematic diagram of PERV structure. (B) Three sgRNAs targeting the gag region. (C) Four sgRNAs targeting the pol region. The sgRNA target sequences are in blue. The objective C base is marked in red. The target codons are boxed in a yellow frame. (D) Structure of multi-target all-in-one Or-iP/EBNA1-based CBE vector.