Figure 3.
FUT2 boosts the early stage of autophagy in lung adenocarcinoma cells. (A) Representative Western blot showing that knockdown of FUT2 reduced the expression of LC3-II induced by serum starvation in A549 cells. (B) Representative Western blot showing that the knockdown of FUT2 reduced the expression of LC3-II induced by NH4Cl in A549 cells. (C) Representative image of immunofluorescence staining showing the stubRFP-sensGFP-LC3 double-fluorescence system. (D) Representative image of immunofluorescence staining showing that the knockdown of FUT2 decreased both autophagosomes (yellow dots) and autolysosomes (free red dots). (* p < 0.05, and ** p < 0.01).