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. 2022 Dec 15;14(24):6206. doi: 10.3390/cancers14246206

Figure 1.

Figure 1

CVB3 induces HT-29 and HCT-116 cells death. HT-29 cells were infected with CVB3 (MOI = 1) for different periods of time. (A) Infected cells were observed using an inverted fluorescence microscope. Propidium iodide (PI) was added at 0, 12, and 24 h after HT-29 infection with CVB3. Magnification, ×200. Scale bar, 50 μm. (B) After CVB3 infection, HT-29 cells were collected at 0, 6, 12, 18, and 24 h and stained with Annexin V-FITC and PI for flow cytometry. Numbers represent the proportion of cells in each quadrant. Cell viability was determined using a CCK-8 assay. HCT-116 cells were infected with CVB3 (MOI = 1) for different periods of time. (C) Infected cells were observed using an inverted fluorescence microscope. Propidium iodide (PI) was added at 0, 24, and 48 h after HCT-116 infection with CVB3. Magnification, ×200. Scale bar, 50 μm. (D) After CVB3 infection, HCT-116 cells were collected at 0, 12, 24, 36, and 48 h and stained with Annexin V-FITC and PI for flow cytometry. Numbers represent the proportion of cells in each quadrant. Cell viability was determined using a CCK-8 assay. The data are expressed as the mean ± standard deviation of three independent experiments. ** p < 0.01, *** p < 0.001, **** p < 0.0001.