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. 2022 Dec 15;14(24):6206. doi: 10.3390/cancers14246206

Figure 5.

Figure 5

The caspase-3-specific inhibitor Z-DEVD-FMK and ASK1 inhibitor selonsertib inhibit the cleavage of GSDME. HT-29 cells were pretreated with or without Z-DEVD-FMK and selonsertib for 2 h and then infected with CVB3 for 24 h. (A,E) Representative brightfield images. Magnification, ×200. Scale bar, 50 μm. (B,C,F,G) CCK-8 and LDH assays for the detection of HT-29 cell viability and cytotoxicity after CVB3 infection. (D,H) Western blots to analyze GSDME and GSDME N terminus, PARP and cleaved PARP, and pro- and cleaved caspase-3/9 in HT-29 cells. * p < 0.05, ** p < 0.01, *** p < 0.001.