TABLE 1.
Cell viability in cocultures of PHA-preactivated T cells and C. trachomatis-infected macrophages
| Cultivation of PHA-stimulated T cells plus: | % of viable cellsa | Pb |
|---|---|---|
| Noninfected macrophages | 100 | |
| Noninfected macrophages plus PHA | 93 ± 19 | 1.0 |
| C. trachomatis (MOI = 5) without macrophages | 90 ± 7 | 0.24 |
| Macrophages infected with C. trachomatis at an MOI of: | ||
| 1 | 86 ± 5 | 0.27 |
| 5 | 67 ± 10 | 0.007 |
| 10 | 69 ± 11 | 0.006 |
a
Values, quantified by FACS analysis of PI-stained cells after 4 days of cocultivation, are means ± standard deviations of data from four independent experiments, each performed in duplicate. Percentages represent the viability of cells under the different culture conditions compared to that of cells in noninfected cultures.
b
P values of the different culture conditions versus cultures with noninfected macrophages, as determined by one-way ANOVA accounting for multiple comparisons by using the Bonferroni test. P in boldface are significant.