TABLE 2.
Induction of T-cell death by supernatant and cell-cell contact of C. trachomatis-infected macrophages
| Cultivation of PHA-activated T cells plus: | % of viable cellsa (Pb) after induction with:
|
|
|---|---|---|
| Supernatant | Cells | |
| Noninfected macrophages | 100 | 100 |
| Macrophages infected with C. trachomatis at an MOI of: | ||
| 1 | 87 ± 9 (0.005) | 84 ± 12 (0.0001) |
| 5 | 78 ± 8 (0.0001) | 61 ± 9 (0.0001) |
Percentages represent the viability of PI-stained cells under different culture conditions compared to the viability of noninfected cultures. Values are means ± standard deviations (six independent experiments, four performed in duplicate and two performed in triplicate). Supernatants were derived from macrophages after 6 days of culture prior to the addition of T cells. FACS analysis for PI-stained cells was performed after 6 days of culture.
All values were highly significantly different (P < 0.01) from value for preactivated T cells cocultivated with supernatant from uninfected macrophages or from value for preactivated T cells cocultivated with noninfected macrophages, as determined by one-way ANOVA.