Table 1.
The 1st generation commercialized expansion treatments for ACI: number of cells provided for implantation procedure by the commercial provider, expansion methodology and cell density recommended for the ACI procedure.
| Commercial Name (Company) |
No. of Cells Provided | Expansion | Recommended Cell Implantation Density |
|---|---|---|---|
| First ACI procedure by Brittberg and Peterson | 2.5–5 million cells | Monolayer, Flask | - |
| Carticel (Genzyme) |
12 million per vial | - | ≥2 × 106/cm2 defect ≤ 7 cm2 |
| ChondroCelect (TiGenix) |
4 million cells per vial | Monolayer, Flask | 0.8–1 × 106/cm2 |
| Chondrosphere Or Spherox (Co.don) |
200 microliter of isotonic sodium chloride, there are 60 spheroids. (single spheroid contains 200,000 cells) 12 million cells implanted in total |
Initially expanded in Monolayer, then transferred into a suspension culture | 10–70 spheroids/cm2 |