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. Author manuscript; available in PMC: 2022 Dec 22.
Published in final edited form as: FASEB J. 2021 May;35(5):e21606. doi: 10.1096/fj.202100203R

Figure 1. Dietary Na+ depletion induces NKCC1 expression in rat distal colon.

Figure 1.

(A) NKCC1 and Na+, K+-ATPase (NaKα) mRNA abundance measured by qRT-PCR using total-RNA isolated from normal and dietary-Na+ depleted rat distal colon. (B) qRT-PCR-amplified NKCC1 transcript products were resolved on 1.5% agarose gel show a single product of expected size (177 bp) in total-RNA isolated from both normal and dietary Na+-depleted rat distal colon. DNA ladder and corresponding fragment sizes are indicated on the left. (C) Representative Western blot of NKCC1 and β-actin (internal control) protein expression in mucosal homogenates from normal (N) and dietary Na+-depleted (D) rat distal colon. Protein ladder is shown in the extreme left lane, and corresponding molecular weights in kDa are indicated. (D) Densitometry quantitation of NKCC1 protein normalized to β-actin. NKCC1 band intensity was measured and divided by the β-actin band intensity for each sample using FIJI (ImageJ) software. Data were then transformed so that control NKCC1/β-actin values were set equal to 1. Lines and error bars represent means ± SEM, *p<0.01, as determined by Student’s unpaired t-test.