Figure 3.
Detection of VZV in clinical samples. Twenty VZV samples were tested with qPCR, but one (sample 4) tested negative for VZV. The positive control used was the VZV plasmid with a concentration of about 3.62 × 106 copies/µL, and nuclease-free water for the negative control. The specificity of RAA-LF was determined using the DNA of monkeypox virus (MPXV) and vaccinia virus (VACV), and both viruses tested negative for VZV. RAA-LF for VZV detection was also conducted, in triplicate, using the same clinical samples tested with qPCR. Nineteen samples, along with the positive control, tested positive for VZV by RAA-LF with the presence of the test band on the dipstick, while sample 4 only exhibited the control band, conforming to the results of the qPCR.