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. 2022 Dec 16;11(24):4094. doi: 10.3390/cells11244094

Figure 7.

Figure 7

Metformin inhibits the proliferation of RAW 264.7 cells. Metformin protects against apoptosis of RAW 264.7 cells induced by high concentrations of LPS. (A) Cell viability was assessed by CCK8 assay. RAW 264.7 cells were treated with different concentrations of metformin (0, 0.1, 0.5, 1, 5, 10, 20 mM) for 24 h. (B,C) Effects of metformin at various dose on apoptosis of RAW 264.7 cells. No significant differences were detected by quantification via Annexin V-FITC/PI flow cytometry. (D,E) Effects of pretreatment of RAW 264.7 cells with different concentrations of metformin on apoptosis induced by LPS (10 μg/mL). The percentages of apoptotic cells are quantified in the bar graph. All experiments were repeated three times and means ± SDs of the data are presented. ns: no significance, *** p < 0.001. Met (0 mM): NIH 3T3 cells treated without metformin for 24 hMet, Met (2.5 mM): NIH 3T3 cells treated with 2.5 mM metformin for 24 h, Met (5 mM): NIH 3T3 cells treated with 5 mM metformin for 24 h, Met (10 mM): NIH 3T3 cells treated with 10 mM metformin for 24 h, LPS + Met: NIH 3T3 cells treated with metformin for 16 h, then stimulated with the addition of LPS (10 μg) for 8 h.