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. 2022 Dec 15;11(24):4070. doi: 10.3390/cells11244070

Figure 7.

Figure 7

Oxidative stress and antioxidant response in HUVECs are challenged with 50μg/mL of extracellular histones (native and citrullinated). (A) Relative expression of antioxidant enzymes involved in the detoxification of superoxide (SOD1 and SOD2) and hydrogen peroxide (CAT and GPX1) evaluated by RT-qPCR in HUVECs treated with extracellular histones (native and citrullinated); (B) representative Western Blot showing the protein levels of the antioxidant enzymes CuZnSOD (SOD-1), MnSOD (SOD-2), catalase (CAT), and glutathione peroxidase 1 (Gpx-1); (C) densitometry of 3–5 independent Western blot experiments showing the relative amount of the antioxidant enzymes in each of the analyzed conditions. Data are expressed as the mean ± SEM from 3–5 independent experiments; * p < 0.05, ** p < 0.01, compared to control (histones 0 μg/mL) unless the lines at the top of the columns indicate differences between compared conditions. Abbreviations: ns, nonsignificant p-value; a.u., arbitrary units; r.u., relative units. The lines at the top of the columns indicate differences between compared conditions.