Figure 1.
KLK6 deficiency attenuated tumor growth and metastasis in vivo. 1 × 106 cells of B16F10 (A–C) or LLC (D–F) cells were subcutaneously injected into WT and KLK6−/− mice (n = 8 per group). (A,D) Representative photograph of tumors. Tumor volume (B,E) and tumor weight (C,F) were calculated as described in the Materials and Methods. 5 × 105 cells of B16F10 (G,H) or LLC (I,J) cells were intravenously injected into the tail vein of WT or KLK6−/− mice (n = 8 per group). (G,I) Representative photograph of metastatic lung nodules from WT and KLK6−/− mice. (H,J) The number of metastatic lung nodules was calculated as described in the Materials and Methods. ** p < 0.01 (Student’s t-test). Data are representative of three experiments. +/+, wild-type; −/−, homozygous knockout.