Figure 3.

KLK6 deficiency abolished BMDM-mediated CXCL1 production in cancer cells. Conditioned medium (CM) was collected from WT and KLK6−/− BMDMs. (A) KLK6 protein expression in CM of WT and KLK6−/− BMDMs were analyzed by Western blot. (B,C) B16F10 cells were treated with the CMs of WT and KLK6−/− BMDMs. mRNA (B) and protein (C) levels of CXCL1 and CXCL2 were analyzed by RT-PCR and ELISA, respectively. (D) KLK6 mRNA levels in WT and KLK6−/− BMDMs were analyzed by RT-PCR. (E,F) B16F10 cells were cocultured with WT or KLK6−/− BMDMs. mRNA (E) and protein (F) levels of CXCL1 and CXCL2 were analyzed by RT-PCR and ELISA, respectively. * p < 0.05 (Student’s t-test). Data are representative of three experiments. +/+, wild-type; −/−, homozygous knockout.