Figure 7.

Recombinant KLK6 promoted macrophage-derived TNF-α production via PAR1 in KLK6−/− BMDMs. (A) KLK6−/− BMDMs were treated with rKLK6 at the indicated concentrations for 24 h. The secreted TNF-α levels were measured by ELISA. (B,C) B16F10 cells were treated with the CMs of KLK6−/− BMDMs treated with rKLK6 at the indicated conditions for 24 h. The levels of CXCL1 mRNA (B) and secreted CXCL1 protein (C) were analyzed by RT-PCR and ELISA, respectively. (D) KLK6−/− BMDMs were treated with rKLK6 in the presence or absence of the PAR1 antagonist (100 nM). The secreted TNF-α levels were measured by ELISA. (E) B16F10 cells were treated with the CMs of indicated KLK6−/− BMDMs for 24 h. The secreted CXCL1 levels were measured by ELISA. ** p < 0.01 and * p < 0.05 (Student’s t-test). Data are representative of three experiments.