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. 2022 Dec 8;11(24):3968. doi: 10.3390/cells11243968

Figure 1.

Figure 1

Phenotype of conditional Lrp5, Lrp6, and double knockout mice. (A) Agarosegel image of PCR genotyping demonstrates the heterozygous and homozygous floxed alleles of Lrp5 and Lrp6. (B) Images of X-ray exposure of Western blot membrane of muscle lysates (GAPDH) or immunoprecipitations of Lrp5 or Lrp6 using muscle hind limb lysates of control and mutant mice as indicated. (C) The graph reflects the body weight of control and single knockout mice at adulthood (4–7 months of age). Note that in comparison with controls, body weight of conditional Lrp6 knockouts is slightly reduced (n = 8–16 mice per genotype). (D) Muscle force is presented by a graph summarizing force per weight for control and conditional single knockout mice. (E) Distribution of offspring of conditional Lrp5 and Lrp6 knockout mice (n = 8–16 mice per genotype). Note that while conditional single knockout mice are born in agreement with mendelian distribution, double knockout die at embryonic stage. All labeling of graph bars represents genotype of mice as indicated in (D). * p < 0.05.