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. 2022 Dec 9;11(24):3983. doi: 10.3390/cells11243983

Figure 4.

Figure 4

Relative mRNA levels, determined by RT-qPCR, of ACOX2 (A), BAAT (B), ABCD3 (C), AMACR (D), HSD17B4 (E), and SCP2 (F) in HepG2 cells, which were incubated for 24 h with the FXR agonists GW4064 (1 µM) or chenodeoxycholic acid (CDCA, 50 µM), the LXR agonist GW3965 (1 µM), the PPAR agonist bezafibrate (80 µM), the HNF4α inhibitor BI6015 (10 µM), or human recombinant FGF19 (50 ng/mL) alone or combined with the MEK1/2 inhibitor U0126 (5 µM). Results are expressed as a percentage of the values determined in untreated cells (Control) and represent the mean ± SD from at least three determinations that were carried out in three different cultures. *, p < 0.05, as compared to Control by paired t-test. †, p < 0.05, as compared with cells incubated with FGF19 alone by paired t-test.