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. 2022 Dec 12;11(24):4021. doi: 10.3390/cells11244021

Figure 4.

Figure 4

Inhibition of tumor cell growth by tumor cell-intrinsic HVEM. (A,E) The CTG assay assessing the cell proliferation of NCI-H1299 (left) and A549 (right) cells transfected with the indicated shRNAs (A) or plasmids (E) (n = 3). (B,F) Immunoblot analysis of the indicated proteins in cells transfected with the indicated shRNAs (B) or plasmids (F). (C,G) Representative images of colony formation for NCI-H1299 (top) and A549 (bottom) transfected with the indicated shRNAs (C) or plasmids (G) (n = 3). (D,H) Quantification data of colony formation for NCI-H1299 (left) and A549 (right) cells transfected with the indicated shRNAs (D) or plasmids (H) (n = 3). Data are presented as the mean ± SD from three independent experiments. * p < 0.05, ** < 0.01, *** < 0.001.