FIG. 4.

Intracellular survival of capsulated parent strain K50 and its noncapsulated variant K50/3. Monolayers of HCT-8 cells were infected with the indicated K. pneumoniae strains. After invasion, the medium was replaced with fresh medium containing 10 μg of gentamicin ml, and the monolayers were lysed at the indicated times to determine the number of the intracellular bacteria expressed as a percentage of inoculum. The asterisk denotes a value significantly different from that obtained after 2 h (P < 0.01). Data shown represent mean values and standard deviations.