Table 1.
The ACE-inhibitory activity, antioxidant activities, protein content, and yield of ultrafiltration fractions obtained from Al-HB.
| Fraction | MW (kDa) | ACE-Inhibitory Activity (IC50, mg/mL) |
Hydroxyl Radical Scavenging Activity (EC50, mg/mL) |
Reducing Power * (Abs at 700 nm) |
Metal-chelating Activity (EC50, ug/mL) |
Protein Content (mg/g) |
Yield |
|---|---|---|---|---|---|---|---|
| HB-I | >5 | 1.19 ± 0.17 a | 6.97 ± 0.70 a | 0.352 ± 0.039 a | 156.23 ± 18.00 a | 104.34 ± 2.37 a | 25.10 ± 0.78 a |
| HB-II | 3–5 | 0.39 ± 0.06 b | 6.74 ± 0.71 a | 0.264 ± 0.010 b | 83.24 ± 9.99 b | 83.25 ± 2.08 b | 19.87 ± 0.21 b |
| HB-III | 1–3 | 0.44 ± 0.10 b | 5.90 ± 0.60 a | 0.203 ± 0.024 b | 34.34 ± 3.20 c | 77.15 ± 4.01 b | 19.27 ± 0.32 b |
| HB-IV | <1 | 0.28 ± 0.03 b | 3.48 ± 0.14 b | 0.116 ± 0.007 c | 34.07 ± 2.24 c | 37.41 ± 0.30 c | 14.37 ± 0.57 c |
| PeptACE ^ | 0.22 ± 0.03 b | 0.44 ± 0.02 c | 0.336 ± 0.024 a | >1000 | ND | ND | |
| Ascorbic acid | ND | 1.57 ± 0.20 c | 0.928 ± 0.027 # | ND | ND | ND | |
| EDTA | ND | ND | ND | 4.34 ± 0.43 d | ND | ND |
Values are mean ± SD, n = 3. Means without a common superscript letter (a–d) within the same column indicate significant difference at p < 0.05. * Sample concentration used for analysis was 1 mg/mL. ^ An antihypertensive supplement composed of fish peptides manufactured by Natural Factors Nutritional Product Ltd. (Coquitlam, Canada). # Ascorbic acid concentration used for reducing power determination was 0.005 mg/mL. ND: not determined.