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. 2021 Jul 12;27(18):5072–5083. doi: 10.1158/1078-0432.CCR-21-2051

Figure 2.

Figure 2. MICSSS/mIF image analysis algorithm harmonization. A, Representative illustration of image analysis algorithm harmonization on prestained mIF images. Images shown are for illustrative purposes to demonstrate workflow only and are not intended as primary data figures. B, Concordance plot of CD3, CD8, PD-1, PD-L1, and cytokeratin log2 transformed cell density data between three centers within each tissue type. The distribution of each marker is shown separately. On the diagonals, box plots are shown to display the values for each sample (HN1-14 and HN2-13) measured at each site (ISMMS, DFCI, and MDACC), respectively. Off diagonals, pairwise scatter plots are shown to illustrate the concordance between two sites. The two samples are coded by red and blue, respectively. All Spearman correlation coefficients are greater than 0.7 except one, which is between ISMMS and MDACC for CD3. We observe that all median Spearman correlation coefficients are greater than or equal to 0.7.

MICSSS/mIF image analysis algorithm harmonization. A, Representative illustration of image analysis algorithm harmonization on prestained mIF images. Images shown are for illustrative purposes to demonstrate workflow only and are not intended as primary data figures. B, Concordance plot of CD3, CD8, PD-1, PD-L1, and cytokeratin log2 transformed cell density data between three centers within each tissue type. The distribution of each marker is shown separately. On the diagonals, box plots are shown to display the values for each sample (HN1-14 and HN2-13) measured at each site (ISMMS, DFCI, and MDACC), respectively. Off diagonals, pairwise scatter plots are shown to illustrate the concordance between two sites. The two samples are coded by red and blue, respectively. All Spearman correlation coefficients are greater than 0.7 except one, which is between ISMMS and MDACC for CD3. We observe that all median Spearman correlation coefficients are greater than or equal to 0.7.