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. 2000 Dec;68(12):6758–6762. doi: 10.1128/iai.68.12.6758-6762.2000

TABLE 1.

Oligonucleotide sequences used in PCR

Oligonucleotide ORF locationb Sequence
ORF1-1 ORF upstream of the area encoding the SspB-binding protein (bp 139127–139147) 5′-GTA GAA GAT GGT GGA ATA ATG-3′
ORF2-1 ORF encoding the SspB-binding protein (bp 140031–140050) 5′-GGT GCT CAA TGA CAT CGC AG-3′
ORF2-2a ORF encoding the the SspB-binding protein (bp 141143–141163) 5′-GAT TGT TAG GAT CCG GAT CCG-3′
ORF3-2a ORF downstream of the area encoding the SspB-binding protein (bp 141963–141982) 5′-GGA TGA GGC AGA CTA TCT AC-3′
a

Antisense oligonucleotide. 

b

Base pair location from the TIGR database (http://www.tigr.org).