TABLE 1.
Oligonucleotide sequences used in PCR
Oligonucleotide | ORF locationb | Sequence |
---|---|---|
ORF1-1 | ORF upstream of the area encoding the SspB-binding protein (bp 139127–139147) | 5′-GTA GAA GAT GGT GGA ATA ATG-3′ |
ORF2-1 | ORF encoding the SspB-binding protein (bp 140031–140050) | 5′-GGT GCT CAA TGA CAT CGC AG-3′ |
ORF2-2a | ORF encoding the the SspB-binding protein (bp 141143–141163) | 5′-GAT TGT TAG GAT CCG GAT CCG-3′ |
ORF3-2a | ORF downstream of the area encoding the SspB-binding protein (bp 141963–141982) | 5′-GGA TGA GGC AGA CTA TCT AC-3′ |
Antisense oligonucleotide.
Base pair location from the TIGR database (http://www.tigr.org).