Proliferation of four representative human T-cell clones specific for MPF2. Each clone was incubated with autologous irradiated PBMC as APC alone or in the presence of PHA, MPF2, native CaMp65, recombinant CaMp65, or recombinant ScMp65. After 2 days of culture, clones were incubated with [3H]thymidine and harvested 18 h later. Results are expressed as the mean counts per minute of triplicates.