TABLE 1.
Sequence and localization of Ca oligonucleotides used for molecular cloning of CaMP65 and Scw10
| Oligonucleotide | Sequencea | Gene | Positionsb |
|---|---|---|---|
| Ca33 | CCCGGATCCATGCGTTTTTCAAATTTCC | Scw10 | 1–19 |
| Ca34 | CCCAAGCTTAATCACTTGATAGAATACC | Scw10 | 1168–1150 |
| Ca64 | AACGGATCCATGTTATTCAAGTCTTTC | CaMP65 | 1–18 |
| Ca65 | GGGCTGCAGGTGCTTAGTTAGAGTAA | CaMP65 | 1144–1128 |
a
The underlined sequences are flanking noncomplementary regions containing the restriction site (in boldface) used to clone the gene in the expression vector.
b
For both primers, position 1 is the first base of the start codon and position 1168 and 1140 are the third base of the stop codons of S. cerevisiae Scw10 and C. albicans MP65, respectively.