TABLE 2.
PBMC proliferation induced by natural Mp65 of C. albicans and recombinant proteins of C. albicans and S. cerevisiae
| Antigenic or mitogenic stimulant | Concn (μg/ml) | PBMC proliferationa (mean cpm ± SD)
|
||
|---|---|---|---|---|
| Donor 1 (high responder) | Donor 2 (intermediate responder) | Donor 3 (low responder) | ||
| None | 1,000 ± 600 | 300 ± 70 | 1,400 ± 600 | |
| PHA | 3 | 35,600 ± 1,700 | 7,400 ± 3,400 | 26,400 ± 3,800 |
| SAMb | 1 | 116,100 ± 10,900 | 23,600 ± 1,400 | 41,400 ± 16,000 |
| 0.1 | 45,600 ± 26,200 | 15,000 ± 3,300 | 8,200 ± 1,000 | |
| Mp65 (native)c | 1 | 123,300 ± 400 | 13,500 ± 800 | 12,400 ± 2,800 |
| 0.1 | 103,400 ± 5,900 | 7,900 ± 800 | 3,300 ± 2,400 | |
| 0.01 | 9,100 ± 4,700 | 800 ± 300 | 1,800 ± 900 | |
| CaMp65d | 1 | 108,600 ± 6,600 | 28,500 ± 3,300 | 9,700 ± 4,900 |
| 0.1 | 51,300 ± 5,100 | 24,300 ± 2,000 | 3,900 ± 2,400 | |
| 0.01 | 5,500 ± 660 | 900 ± 100 | 1,100 ± 200 | |
| ScMp65d | 1 | 7,600 ± 4,300 | 8,500 ± 5,800 | 2,500 ± 900 |
| 0.1 | 4,900 ± 4,700 | 3,000 ± 1,400 | 600 ± 300 | |
| 0.01 | 1,200 ± 400 | 500 ± 200 | ||
a
Evaluated as [3H]thymidine incorporation and expressed as counts per minute. The PBMC donors were arbitrarily classified as high, moderate, and low responders as described in Materials and Methods.
b
SAM is the secretory mannoproteic material from hyphal cells (see reference 4), with concentration expressed as polysaccharide.
c
Affinity chromatography purified (20, 21), with concentration expressed as protein.
d
Recombinant proteins produced in E. coli and purified by Ni-NTA affinity chromatography.