Schematic diagram of molecular structure, genome organization, and relative positions of amplicon targets on the SARS-CoV-2 genome. On the SARS-CoV-2 virion, together with membrane protein and envelope protein, the spike protein glycoprotein projects from a lipid bilayer, giving the virion a distinctive appearance. SARS-CoV-2 virion (top): M: membrane protein; E: envelope protein; S: spike protein; N: nucleocapsid protein. The SARS-CoV-2 genomic RNA is associated with the nucleocapsid protein forming the ribonucleoprotein with a helical structure. The SARS-CoV-2 genome encodes structural (S, M, E, N) and nonstructural proteins. The relative positions of amplicon targets (RdRp, E, N) are shown. SARS-CoV-2 genome (bottom): Orf1a: open reading frame 1a; Orf1ab: open reading frame 1ab; S: spike protein gene; M: membrane protein gene; RdRp: RNA-dependent RNA polymerase gene; E: envelope protein gene; N: nucleocapsid protein gene (adapted from Corman et al. [3]; Artika et al. [55]).