TABLE 5.

Immunogenic proteins identified in the culture supernatants of GAS strains MGAS 315 and MGAS 5005

Spot no.	Identical or homologous protein	Antibody ina:
		Mouse sera		Human sera
		MGAS 315	MGAS 5005	Pharyngitis	ARF	STSS
8	GroEL	+	+	+	+	+
12	Phosphoglycerate kinase	+	+	−	−	−
13	Immunogenic secreted protein homologue	+	+	+	+	+
14	Streptolysin O	+	+	+	+	+
15	M3 protein	+	−	−	−	−
16, 33	NAD+-glycohydrolase	+	+	+	+	+
18	Glyceraldehyde-3-phosphate dehydrogenase	−	−	−	+	−
22	Human Mac-1	+	+	+	+	+
24	Serine protease	+	+	+	+	+
26	Streptococcal pyrogenic exotoxin A	+	−	−	−	+
30	Cyclodextrin glucosyltransferase	−	+	−	−	−
31, 34, 40	M1 protein	−	+	−	+	+
35	L. lactis 45-kDa secreted protein	+	+	+	+	+
36	Streptococcal inhibitor of complement	−	+	−	−	−
38, 39	Mitogenic factor (DNase)	+	+	+	+	+
41	Streptokinase	+	+	+	+	+

^aDetection of antigen-antibody reactivity for each protein was based on representative Western blot data, as represented in Fig. 3 to 5. The Western blot results were obtained with sera from mice infected subcutaneously with MGAS 315 or MGAS 5005 and from humans with pharyngitis, acute rheumatic fever (ARF), or streptococcal toxic shock syndrome (STSS). +, antibody reactivity detected; −, no antibody reactivity detected.