Figure 4.

Time-course of W. anomalus ME1 strain growth by daily addition of gliadin proteins. (a) Growth of the W. anomalus ME1 strain (WaME1) and the baker’s yeast (Sc) in unfed (SMW) and fed (SMW + PGli or SMW + GGli) media. Synthetic media were fed daily with gliadins (0.5% v/v) from day 1 to day 6, both included. The points represent the average OD₆₀₀ ± standard deviation daily of three independent experiments. (b) SDS-PAGE analysis of protein content in fed and unfed cultures. Samples were obtained by adding 10% trichloroacetic acid to 5 mL of culture after removing yeast cells by centrifugation. The protein pellet was resuspended in 25 µL of loading buffer, and half the sample (12.5 µL) was loaded onto the gel. The analysis of the samples taken from W. anomalus (Wa) cultures on days 1, 6, and 7 (d1, d6, d7) and from S. cerevisiae (Sc) cultures on day 6 (d6), just before adding the daily dose of gliadin proteins, is shown. M, protein mixture (25–260 kDa) was used as the molecular weight marker.