Figure 1.

Thiolated gelatin (Gel-SH) functionalization. (A) Gel-SH synthesis schematic. Gel-SH was synthesized through 1-ethyl-3(3-dimethylamino)propyl carbodiimide (EDC)/N-hydroxysuccinimide (NHS) conjugation of L-cysteine to the native fish gelatin backbone. (B) Amine content of Gel-SH determined via TNBS assay (mean ± SEM; n = 3); (C) Thiol content of Gel-SH determined via DTNB assay (mean ± SEM; n = 3); (D) ¹H-NMR of Gel-SH and native gelatin. Gelatin thiolation indicated by increase in signal at peaks (a), (b) and (d), and decrease in signal (c) in Gel-SH compared to native gelatin.