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. 2022 Dec 12;8(12):821. doi: 10.3390/gels8120821

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Osteogenic differentiation of patient-derived preosteoblasts in Gel-SH/PEG-4MAL hydrogels. (A) Day 28 alizarin red staining (ARS) of patient-derived preosteoblast-laden Gel-SH/PEG-4MAL hydrogels cultured using either α-MEM growth media (GM) or osteogenic media (OM). n = 6; (B) Day 28 macroscopic images of ARS-stained, patient-derived preosteoblast-laden Gel-SH/PEG-4MAL hydrogels; (C) DNA content of patient-derived preosteoblasts encapsulated in Gel-SH/PEG-4MAL hydrogels over time, normalized to hydrogel weight and day 1 DNA content (n ≤ 5; mean ± SEM).