β-gal+ cell counts in the Prelimbic Cortex (a), Infralimbic Cortex (b), Dorsal Agranular Insular Cortex (c), Ventral Agranular Insular Cortex (d), Nucleus Accumbens Core (e), Nucleus Accumbens Shell (f), Dorsal Striatum (g), Basolateral Amygdala (h), and Central Amygdala (j) in adolescent and adult males previously conditioned with 0, 1.5 or 2.5 g/kg ethanol on test day following exposure to the CS (NaCl). (i) Images of β-gal+ cells in AIV of adult rats, 1mm in length. n = 7-11/group; # denotes main effect of age, asterisks (*) mark significant ethanol-associated changes relative to controls conditioned with with saline (0 g/kg ethanol dose) within each Age (p < 0.05).