TABLE 1.
Proliferation and cyokine profiles of Mycobacterium-specific T-cell lines
| T-cell line | Results obtained in the presence of:
|
Profile | |||||
|---|---|---|---|---|---|---|---|
| MIMa
|
HKMb
|
||||||
| SIc | Leveld (pg/ml) of:
|
SIc | Leveld (pg/ml) of:
|
||||
| IFN-γ | IL-4 | IFN-γ | IL-4 | ||||
| Mtb.90 | 23 | 236 | <5 | 10.6 | 236 | <5 | Th1 |
| Mtb.94 | 15 | 19 | 67 | 7.6 | <10 | 8 | Th2 |
| Mtb.101 | 14 | 157 | 20 | 9.2 | 813 | <5 | Th1 |
| Mtb.102 | 28 | 331 | 37 | 8.5 | 54 | <5 | Th1 |
| Mtb.104 | 10.4 | 193 | <5 | 3.2 | 85 | 18 | Th1 |
| Mtb.106 | 33.5 | 215 | <5 | 10.2 | 528 | <5 | Th1 |
| Mtb.107 | 12.5 | 597 | 37 | 8.9 | 512 | <1 | Th1 |
| Mtb.108 | 8.0 | 50 | <5 | ND | <5 | 12 | Th1 |
| Mtb.184 | 5.1 | 600 | <5 | 4.0 | 644 | <5 | Th1 |
| Mtb.186 | 6.1 | 116 | <5 | 5.2 | 264 | <5 | Th1 |
| Mtb.187 | 19.4 | 30 | 7 | 23.2 | 91 | 5 | Th1 |
a
MIM ratio, 20:1.
b
HKM (15 μg/ml)-pulsed macrophages.
c
Background counts per minute for SI (mean and standard deviation), 674 ± 434. ND, not determined.
d
Supernatants were harvested from antigen-stimulated and nonstimulated cultures (T cells plus normal macrophages) and tested for cytokines. Data are reported as levels of cytokines from stimulated cultures minus unstimulated cultures. The amount of IFN-γ produced from unstimulated cultures was 1.6 ± 5.1 pg/ml (mean and standard deviation; 95% confidence interval upper limit, 5.1), and that of IL-4 was 7.7 ± 19.4 pg/ml (95% confidence interval upper limit, 20).