TABLE 6.

Immunization with rAg85B induces Mycobacterium-specific human T-cell responses^a

T-cell line	Results obtained in the presence of:
	MIM		HKM		rAg85B
	SI	IFN-γ level	SI	IFN-γ level	SI	IFN-γ level
N-85B.149	5.0	<5	2.0	71	2.0	<5
T-85B.149	3.2	40	2.0	223	2.1	92
N-85B.159	1.0	62	ND	ND	3.8	240
T-85B.159	2.0	190	ND	ND	4.6	641
N-85B.173	ND	43	ND	61	ND	<5
T-85B.173	ND	864	ND	3,106	ND	336
N-85B.186	10.0	<5	10.7	<5	4.5	12
T-85B.186	6.8	<5	10.4	31	15	107
N-85B.187	10.7	5	3.6	<5	6.6	<5
T-85B.187	3.8	594	1.5	260	2.7	734
N-85B.191	4.2	<5	2.3	<5	2.4	<5
T-85B.191	3.3	50	2.3	36	2.3	48

^aResting cells from rAg85B-specific T-cell lines were stimulated in vitro with MIM (20:1) or HKM (15 μg/ml)- or rAg85B (10 μg/ml)-pulsed normal macrophages for 3 days. Supernatants were harvested and tested for the IFN-γ concentration (picograms per milliliter) by an ELISA. Proliferation was assessed by measuring ³H-thymidine incorporation. ND, not determined. T-cell lines designated with “N” were generated by immunization of normal PBMC with rAg85B. T-cell lines designated with “T” were generated by immunization of normal PBMC with rAg85B plus IL-12 (1 ng/ml).