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. 2022 Dec 7;23(24):15452. doi: 10.3390/ijms232415452

Figure 5.

Figure 5

Identification of transgenic hairy roots of S. baicalensis. (A) Transgenic hairy root lines and observations with red fluorescent protein. A4: ArA4 lines (negative control); EV: ArA4 strain that harbors the pK7WG2R plasmids (positive control); MYB12-1~3: SbMYB12 overexpression hairy root lines. (B) PCR identification of transgenic hairy roots of S. baicalensis. PCR screening of SbMYB12 overexpressing lines. M: DNA marker (DL2000); P: ArA4 strain containing recombinant plasmid (positive control); N: S. baicalensis sterile plantlet (negative control); W: wild seedling of S. baicalensis. (C) Real-time quantitative PCR analysis of SbMYB12 in transgenic lines. CK1-3: ArA4 lines; OE1–3: SbMYB12 overexpression transgenic line; ** represents significant difference (p < 0.01) via Student’s t-test.