Table 1.
List of Gene Constructs Used to Test Functional Effects of Amino Acid Mutations and Species-Specific Insertions in ATP1A1 of the Common Chinchilla (Chinchilla lanigera) and Yellow-Throated Sandgrouse (Pterocles gutturalis).
| Construct Name | Engineered substitution | AA state at 111 | AA state at 122 | Insertion state |
|---|---|---|---|---|
| *Chinchilla | None | E111 (wt) | N122 (wt) | insM (wt) |
| Chinchilla–insM | −insM | E111 (wt) | N122 (wt) | None (mut) |
| *Chinchilla + N122D | N122D | E111 (wt) | D122 (mut) | insM (wt) |
| Chinchilla + N122D–insM | N122D −insM | E111 (wt) | D122 (mut) | None (mut) |
| *Sandgrouse | None | R111 (wt) | N122 (wt) | insD (wt) |
| Sandgrouse–insD | −insD | R111 (wt) | N122 (wt) | None (mut) |
| *Sandgrouse + R111Q | R111Q | Q111 (mut) | N122 (wt) | insD (wt) |
| Sandgrouse + R111Q–insD | R111Q −insD | Q111 (mut) | N122 (wt) | None (mut) |
Note.—The corresponding wildtype ATP1B1 gene of each recombinant protein construct was co-expressed with ATP1A1. Asterisks indicate constructs generated by Mohammadi et al. (2022). The abbreviations “wt” and “mut” indicate wildtype or derived states, respectively.