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. 2022 Dec 9;23(24):15591. doi: 10.3390/ijms232415591

Figure 1.

Figure 1

(a) Coomasie-stained BN-PAGE gels loaded with 293T and 293T EncA_PAmCherry cell lysates. Arrow and dotted line indicate the band corresponding to Mx EncA protein shells; (b) 293T EncA_PAmCherry and (c) 293T cells stained with Alexa Fluor 488 anti-DYKDDDDK Tag antibody (green fluorescence signal). Nuclei were counterstained with DAPI (blue fluorescence signal). Laser scanning confocal microscopy, Nikon Eclipse Ti2, scale bars are 50 μm; (d) Influence of the presence of a genetically encoded label on viability and proliferation of 293T EncA_PAmCherry cells. Optical density is proportional to the number of living cells. The numbers of living cells were not significantly different in 293T EncA_PAmCherry and 293T cells. The data are shown as the mean + S.D. of three independent experiments, p values were calculated using a one-tailed t-test, assuming unequal variances.