Skip to main content
. 2022 Dec 7;23(24):15508. doi: 10.3390/ijms232415508

Figure 7.

Figure 7

Metformin suppresses inflammation via the AMPK/mTOR signaling pathway. (a) TAO-OFs were pretreated with metformin for 24 h and then stimulated with 1 ng/mL of IL-1β for 24 h. The expression levels of inflammation-associated proteins were determined by Western blotting. (b) Statistical analysis of the results in (a). (c) The protein levels of AMPK, p-AMPK, mTOR, p-mTOR, p70S6K, p-p70S6K, p65, p-p65, p38, p-p38, AKT, and p-AKT in cells pretreated with metformin, AICAR, and CC before stimulation with IL-1β for 3 h were examined by Western blotting. (d–i) Quantification of the results in (c). The data are presented as the means ± SDs. n = 3 in each group. ### p < 0.001, #### p < 0.0001, compared with the control group, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared with the stimulated group, ns: not significant (ANOVA).