TABLE 1.

Anaerobically grown C. albicans cells release ATP in response to Hst 5 but are protected from killing^a

Growth	Treatment	Killing (%)b	ATP (pmol/106 cells)b
			Extracellular	Intracellular
Aerobic	Control	0	0.4 ± 0.2	56 ± 8
	Hst 5	97 ± 2	99 ± 34	1.1 ± 0.3
Anaerobic	Control	0	0.4 ± 0.3	49 ± 6
	Hst 5	40 ± 4	86 ± 18	4.8 ± 4

^aC. albicans (10⁶ cells, strain DS1) was grown aerobically or anaerobically in synthetic medium containing Oxyrase. Cells were treated for 1.5 h at 37°C with 31 μM Hst 5. Cell supernatants were then assayed for released ATP, whereas the cell pellets were used to assess viability and for determination of intracellular ATP. Extracellular and intracellular ATP levels were measured by luminometry, and loss of viability was assayed as described in the legend to Fig. 1. 

^bValues are means and standard deviations from duplicates from four independent experiments.