TABLE 2.

Pharmacological evidence for the presence of candidal cytotoxic ATP receptors^a

Treatment	Loss of cell viability (%)b
	Without Hst 5	With Hst 5
Control	0	100
ATP4−	68 ± 6
BzATP	61 ± 4
ATPγS	62 ± 6
2MeSATP	20 ± 2
β,γ-MeATP	16 ± 6
ADP	9 ± 3
AMP	14 ± 8
Adenosine	5 ± 3
Apyrase	6 ± 6	54 ± 8

^aC. albicans cells (strain DS1) were incubated for 3 h at 37°C with the indicated nucleotide receptor agonists (ATP⁴⁻, BzATP, and ATPγS at 100 μM; 2MeSATP, β,γ-MeATP, ADP, AMP, and adenosine at 500 μM), or cells were mixed with Hst 5 (31 μM) and the ATP scavenger apyrase (40 U/ml) was included during the 1.5-h incubation at 37°C. Loss of viability was assayed as described in the legend to Fig. 1. 

^bValues are means and standard deviations of duplicate determination from seven (BzATP and ATPγS), three (2MeSATP, ADP, AMP, adenosine, and apyrase) or two (β,γ-MeATP) independent experiments.