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Thermal unfolding of shortened CoVS-HR1 miniproteins. (a,b) DSC scans of CoVS-HR1-N (a) and CoVS-HR1-C (b) measured at varying pH values as indicated next to each curve. The protein concentration was 30 μM in all experiments. (c,d) Effect of protein concentration on the DSC thermograms at pH 7.4. Al experiments were recorded at a scan sate of 90 °C h−1. The thermograms are artificially displaced along the vertical axis for the sake of clarity.
