Table 1.
Primers used in the study.
| Gene | Primer | Sequence 5′-3′ |
|---|---|---|
| Flag-TRIB3 | Sense | CGCGGATCCGCCACCATGGATTACAAGGATGA |
| CGACGATAAGCGAGCCACCCCTCTGGCTGCT | ||
| Anti-sense | CCGCTCGAGCTAGCCATACAGAACCACTTCTC | |
| TGTCTCCCTCCTCTTCCCTG | ||
| HA-ATF4 | Sense | CGCGGATCCGCCACCATGACCCATACGACGTC |
| CCAGACTACGCTACCGAAATGAGCTTCCTGAG | ||
| Anti-sense | CCGCTCGAGCTAGGGGACCCTTTTCTTCCCCC | |
| TTGCCTTGCGGACCTCTTCT | ||
| shTRIB3 | Sense | CCGGGATCTCAAGCTGTGTCGCTTTCTCGAGA |
| AAGCGACACAGCTTGAGATCTTTTTG | ||
| Anti-sense | AATTCAAAAAGATCTCAAGCTGTGTCGCTTTCT | |
| CGAG AAAGCGACACAGCTTGAGATC | ||
| ATF4 (TSS-1000 bp to +100 bp) |
Anti-sense | CGGGGTACCTTCTGTGGCAGCCTTGCACTTGAG |
| CCGGATGAAAATTGTAAAAACCC | ||
| Sense | CCCAAGCTTCCCCTAATACGCCATGGTGGCCG | |
| TGGACCCTGAGGGCGGGGAGGAGG | ||
| ATF4 (TSS-100 bp to +1000 bp) |
Sense | CGGGGTACCCGGGAGGAGACGGTCACGTGGT |
| CGCGGCGGAAGGATGCGTCTGTGCT | ||
| Anti-sense | CCCAAGCTTCCTCCAGGTAATCATCTAAGAG | |
| ACCTAGGCTTTCTTCAGCCCCCAAA |