FIG. 4.

Colony morphology of wild-type smooth and rugose variants of the N16961 strain. The left panel shows the streak plates, and the right panels show the magnified individual colonies on the respective plates. (Top panel) N16961 R, wild-type rugose; N16961 S, wild-type smooth. (Middle panel) NS25 and AA54, hydG::TnphoA and hydG::Kan^r mutants, respectively; AA10, epsD::Kan^r. Only AA54 and AA10 colonies are shown in the middle right panel. The NS25 colonies were similar to the AA54 colonies. (Bottom panel) AA10 complemented by the epsD⁺ plasmid pDSK-2 and NS25 and AA54 complemented by hydG⁺ plasmid. pAA435 is a pWSK29 derivative containing the hydG⁺ gene (SacII-BamHI) from pAA2311 (see the text).