Figure 3.

Expression of HEV/RBD protein in Nicotiana benthamiana plants. Coomassie brilliant blue-stained gel (a) and Western blot (b) of proteins isolated from N. benthamiana plants. M, molecular weight marker (sizes are shown in kD); EL, total proteins isolated from the non-infiltrated leaf; AL, total proteins isolated from leaf infiltrated with pEff_ER-HEV/RBD; PP, purified HEV/RBD protein. Western blotting was performed using anti-RBD antibodies. The position of HEV/RBD protein (calculated molecular weight 81 kD) is shown by an arrow.