Figure 4.

CL of isolated neutrophils (2 million cells/mL) resuspended in plasma (a) and in platelet-rich plasma (PRP) (b). Autologous plasma accounted for 70% of the total solution volume. The blue curves present the CL kinetics for the control sample, the red curves show the CL of a sample incubated with DBPG (1 mg of scaffold per 125 µL of neutrophil suspension) at 36.7 °C. The incubation time is indicated above the CL curves. Blood was added at point time 0 min to the chemiluminometer cuvette containing 485 µL of Krebs–Ringer medium (with NaHCO₃ and CaCl₂) and 200 µM luminol, then 100 nM PMA was added. The x-axis of the graphs represents the time of CL measurement. Measurements were carried out at 37 °C until the CL maximum was reached for all samples. Each CL curve is the average of three curves obtained by measuring of three independent probes taken from one sample (the error did not exceed 15%). Inserts show comparison of CL parameters for the experiments with PPP (difference in CL maximum) and PRP (difference in CL peak time) after 2–5 min and 30–40 min of incubation. Further incubation did not significantly change the ratio of parameters. Data are means ± S.D., n = 3 and 4, ** p < 0.01 vs. Control, *** p < 0.001 vs. Control.