Table 1. List of fly stocks, chemicals and antibody used throughout this study.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Organism/Strains | ||
| D. melanogaster: Canton-S | Bloomington Drosophila Stock Center | BDSC: 64349 |
| D. melanogaster: Oregon-R | Bloomington Drosophila Stock Center | BDSC: 5 |
| D. melanogaster: w1118 | VDRC | FlyBase ID: FBgn0003996 CG2759 |
| D. melanogaster: w[*]; P{w[+mC] = ppk-Gal4.G}3 | Bloomington Drosophila Stock Center | BDSC: 32079 |
| D. melanogaster: y[1] w[*] P{y[+t7.7] w[+mC] = UAS-myrGFP.QUAS-mtdTomato-3xHA}su(Hw)attP8; P{y[+t7.7] w[+mC] = trans-Tango}attP40 | Bloomington Drosophila Stock Center | BDSC: 77124 |
| D. melanogaster: w*,dicer;+;SPRIR | Donated by Carolina Rezával Lab | |
| D. melanogaster: Fru-Gal4/TM3 | Donated by Carolina Rezával Lab | |
| D. melanogaster: dsx-Gal4 | Donated by Carolina Rezával Lab | |
|
D. melanogaster SPSN-SS: w+/w-; VT058873-p65ADZp in attP40/+; VT003280-ZpGal4DBD in attP2/+ Wang et al. 2020 [52] |
Donated by José Duhart | Janelia FlyLight Collection (https://splitgal4.janelia.org/cgi-bin/splitgal4_summary.cgi) |
| D. melanogaster: w[1118]; P{w[+mC] = UAS-DenMark}2, P{w[+mC] = UAS-syt.eGFP}2; In(3L)D, mirr[SaiD1] D[1]/TM6C, Sb[1] | Bloomington Drosophila Stock Center | BDSC: 33064 |
| Antibodies | ||
| Rat polyclonal anti-PDF | Depetris-Chauvin et al., 2011 [53] | N/A |
| Cy-5 AffiniPure Donkey Anti-Guinea Pig IgG | Jackson ImmunoResearch Lab | Cat# 706-175-148 |
| Rabbit polyclonal anti-DsRed | Rockland (Takara) | Cat #632496; RRID: AB_10013483 |
| Chicken polyclonal anti-GFP | Aves Lab | Cat# GFP-1020, RRID: AB_10000240 |
| Chemicals | ||
| KCl, Potassium chloride | Sigma-Aldrich | P3911; CAS: 7447-40-7 (ACS reagent, 99.0–100.5%) |
| NaH2PO4, Sodium phosphate monobasic | Sigma-Aldrich | S8282; CAS: 7558-80-7 (BioXtra, ≥99.0%) |
| D-(+)-Glucose | Sigma-Aldrich | G8270; CAS: 50-99-7 (≥99.5% (GC)) |
| Paraformaldehyde | Sigma-Aldrich | 441244; CAS: 30525-89-4 |
| NaCl, Sodium chloride (for PBS solution) | Cicarelli Laboratorios | 750; CAS: 7647-14-5 |
| Na2HPO4, Sodium phosphate dibasic | Sigma-Aldrich | S3264; CAS: 7558-79-4 (formolecular biology, ≥98.5% (titration)) |
| Triton X-100 | Sigma-Aldrich | T9284; CAS: 9002-93-1 (BioXtra) |
| Goat serum | Natocor | 734 |
| Vetbond Tissue Adhesive | 3M | 1469SB |