Table 2.
Number of samples regenerated after cryopreservation and different thawing treatments. The three treatments were 24 h both in 0.2 M and 0.1 M sugar concentration LM media before the proliferation media (0.03 M sucrose), 24 h in a 0.1 M sucrose concentration before proliferation media and placing samples directly on LM proliferation media (0.03 M sucrose concentration). Three cryovials containing embryogenic tissue from the same genotype from every experimental media were stored in LN.
| SE Line | 0.2 M and 0.1 M Sucrose | 0.1 M Sucrose | 0.03 M Sucrose | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Growing | Slow Growing | Dead | Growing | Slow Growing | Dead | Growing | Slow Growing | Dead | |
| 655 | 3 | 3 | 3 | ||||||
| 809 | 3 | 3 | 2 | 1 | |||||
| 1037 | 3 | 3 | 3 | ||||||
| 1046 | 3 | 2 | 1 | 2 | 1 | ||||
| 1082 | 3 | 3 | 1 | 2 | |||||
| 1119 * | 3 | 2 | 1 | ||||||
| 1129 * | 3 | 3 | |||||||
| 1130 | 3 | 2 | 1 | 3 | |||||
| 2851 | 3 | 3 | 3 | ||||||
| 4262 | 3 | 3 | 3 | ||||||
| 5111 | 3 | 3 | 3 | ||||||
| 5129 | 3 | 3 | 3 | ||||||
| Total | 9 | 18 | 9 | 18 | 9 | 9 | 11 | 4 | 15 |
* 0.03 M sucrose concentration media was not included.